Evaluation of a latex test for rotavirus detection

Evaluation of a latex test for rotavirus detection.

A latex agglutination (LA) test (Slidex Rota-Kit; bioMérieux, Marcy-l'Etoile, France) was a rapid, easily used method for detecting rotavirus (RV) in pediatric fecal specimens. With 45 RV-positive and 50 RV-negative diarrhea specimens, the sensitivity of the LA test was 82%, and the specificity was 100%. Six other specimens produced indeterminate results. The frequency of positive LA tests appe...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test

Development of a slide latex agglutination test for rotavirus antigen detection.

The aim of this study was to optimize the conditions for the passive adsorption of polyclonal antibody onto plain surface polystyrene latex particles and its performance in a slide latex agglutination test for rotavirus antigen detection. Cleaning of latex particles by washing through repetitive centrifuging, decanting and resuspending in distilled water was adequate in removing surfactants fro...

agglutination . Detection of rotavirus by latex

I was very interested to read the letter of Dr Waitkins et al on the importance of Streptococcus milleri. We too isolate the organism from cases of empyema from time to time and would agree about the importance of S milleri in this context. Although we welcome this reminder about the pathogenicity of the organism, there is one aspect of the letter that could be misleading: S milleri apparently ...

Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples.

The performance of the Virogen Rotatest latex agglutination test (LAT) was evaluated for detection of bovine rotavirus antigen. Sixty-three fecal samples from diarrheic calves were collected from November 1999 to May 2000 and screened by LAT, the Rotazyme II enzyme-linked immunosorbent assay (ELISA), and virus isolation (VI) followed by an anti-rotavirus fluorescent-antibody (FA) test to detect...